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  Lysine-N-(N,N-diacetic acid), trifluoroacetic acid salt [NTA]
Catalog Number
100 mg
  Certificate of Analysis
  Material Safety Data Sheet
Comments :
No animal source
Storage :

Protect from light and moisture; Store at 4-8oC

Analytical Data :

≥98% by TLC (ninhydrine dip), soluble in DMSO, DMF, CH3OH and H2O

Synonym :

[L-Lysine, N2,N2-bis(carboxymethyl)-, mono(trifluoroacetate)] ,, [Lysine-Na-(N,N-diacetic acid), trifluoroacetic acid salt] ,, [N-(5-Amino-1-carboxypentyl)iminodiacetic Acid]

Application :

Building block to NTA probes. A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.

References :

1; Hochuli, E., et al.: J. of Chromatography, 411, 177 (1987), Meredith, G.D., et al.: Bioconjugate Chem., 15, 969 (2004).-------2; Adsorption Characteristics of Arsenic(III) and Arsenic(V) on Iron(III)-loaded Chelating Resin Having Lysine-N,N-Diacetic Acid Moiety, Reactive Polymers, 29, 167(1996).

Lysine-N-(N,N-diacetic acid), trifluoroacetic acid salt [NTA]

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