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  4',6-Diamidino-2-phenylindole, dihydrochloride salt [DAPI, 2 HCl ]
Catalog Number
10 mg
  Certificate of Analysis
  Material Safety Data Sheet
Comments :
No animal source
Storage :
Protect from light and moisture; Store at -20oC
Analytical Data :
Exc:360 nm / Em: 462 nm (DAPI/DNA complex),, Soluble in DMSO, CH3OH and H2O
Synonym :
1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, dihydrochloride
Application :
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
References :
1; Kapuscinski, J., DAPI: a DNA-specific fluorescent probe. Biotechnol. Histochem. 70, 220-233, (1995).------2; Otto, F.J., High-resolution analysis of nuclear DNA employing the fluorochrome DAPI. Meth. Cell Biol. 41, 211-217, (1994).------3; Uphoff, C.C., et al., Mycoplasma contamination in human leukemia cell lines. I. Comparison of various detection methods. J. Immunol. Meth. 149, 43-53, (1992).-------4; Collins, J.A., Major DNA fragmentation is a late event in apoptosis. J. Histochem. Cytochem. 45, 923, (1997).
4',6-Diamidino-2-phenylindole, dihydrochloride salt [DAPI, 2 HCl ]

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