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  Pyrene-1,3,6,8-tetrasulfonic acid, tetrasodium salt [HPTS]
Catalog Number
250 mg
  Certificate of Analysis
  Material Safety Data Sheet
Comments :
No animal source
Storage :
Protect from light; Store at room temperature
Analytical Data :
Exc: 375 nm / Em: 404 nm in H2O,, Soluble in H2O,,, ≥98% by HPLC
Synonym :
Pyrene-1,3,6,8-tetrasulfonic acid, tetrasodium salt
Application :
pH-sensitive tracer. It fluoresces blue in acidic solutions and in acidic organelles, but fluoresces green in more basic organelles. And also could be used as a probe for proton translocation,
References :
1; Probing the diphosphoglycerate binding pocket of HbA and HbPresbyterian (beta 108Asn --> Lys)." Gottfried DS, Manjula BN, Malavalli A, Acharya AS, Friedman JM. Biochemistry 38, 11307-11315 (1999).-------2; Determination of ion permeability by fluorescence quenching." Garcia AM. Methods Enzymol 207, 501-510 (1992).-------3; Rapid development of vasopressin-induced hydroosmosis in kidney collecting tubules measured by a new fluorescence technique." Kuwahara M, Berry CA, Verkman AS. Biophys J 54, 595-602 (1988).--------4; On the Intramicellar Fluorescence Quenching Rate Constant in Cylindrical Micelles." Van der Auweraer M, De Schryver FC. Chem Phys 111, 105 (1987).---------5; Channel-mediated monovalent cation fluxes in isolated sarcoplasmic reticulum vesicles." Garcia AM, Miller C. J Gen Physiol 83, 819-839 (1984).
Pyrene-1,3,6,8-tetrasulfonic acid, tetrasodium salt [HPTS]

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